Andaliman fruit extract has been shown to exhibit anti-inflammatory activity, immunostimulant effects, and anticancer activity. It contains antioxidant compounds, which can stimulate the immune system. The purpose of the study was to determine the antioxidant activity of andaliman fruit (Zanthoxylum acanthopodium DC.) using the DPPH method (2,2-diphenyl-1-picrylhydrazyl). The experimental research was conducted in January 2024. The sample used in this study was andaliman fruit (Zanthoxylum acanthopodium DC.) purchased from Onan Rungu village, Samosir Regency, North Sumatra Province. Ethanol extract of andaliman fruit showed significant antibacterial activity against Staphylococcus aureus and Staphylococcus epidermidis at various concentrations, with the largest zone of inhibition at a concentration of 300 mg/mL. These results demonstrate the potential of andaliman as a source of natural antibacterial agents that can be used in the development of health products.