Against the background of new quality productivity, knowledge graphs, and AI technology have opened up new paths for higher education reform, promoting the transformation of traditional teaching models to “smart classrooms.” Taking the “Botany” course as the research object, this paper explores the path of constructing a blended online-offline teaching model using AI + knowledge graphs to address the problems of a fragmented knowledge system, static teaching resources, and a single learning path in traditional teaching. Based on the technological characteristics and educational application value of AI and knowledge graphs, a “Knowledge Graph + AI Scenario Practice + BOPPPS” model is proposed: a knowledge graph is constructed by extracting knowledge points and sorting out knowledge relationships to help students consolidate basic knowledge; scenario-based practice tasks are released through an AI course assistant to stimulate students’ learning interest, and full-process blended teaching activities of “pre-class—in-class—post-class” progressive exploration are carried out, aiming to enhance students’ independent learning, communication, and teamwork abilities. Research results show that this blended teaching model can effectively realize the systematic organization and visual presentation of botanical knowledge, significantly improving students’ learning efficiency and deep learning capabilities.