The Mongolian culture is part of China's traditional culture. It is a prairie culture of fusion Mongolian wisdom for nearly a thousand years. This article briefly introduces the basic concepts of mascots and Mongolian cultural elements, and carries out a design practice by using the horse culture, color matching, boke clothing in the Mongolian cultural elements. The white horse mascot was designed by combining with national culture. It can be found that Mongolian traditional culture is a huge resource treasure house for mascot design through design practice, and the design of mascot is the characteristic of the era that inherits the development of Mongolian culture. The integration of Mongolian culture and mascot design can achieve mutual benefit.