Career values are the specific manifestations of life goals and attitudes in career choices. In the context of the new era, post-2000 vocational college students exhibit characteristics such as diversified and personalized career values, economic value preferences in career choices, and a disconnect between career aspirations and reality. Based on this, this article proposes that the cultivation of vocational values for post-2000 vocational college students includes five aspects: education on socialist core values, education on career development and professional qualities, cultivation of professional spirit, education on the connotation and extension of vocational values, and education on network culture and vocational values. The cultivation path should focus on combining theoretical education with practical experience, creating campus culture and professional atmosphere, personalizing career guidance and career planning, cultivating social responsibility and craftsmanship spirit, etc., in order to meet the demand of society for high-quality technical and skilled talents and provide strong talent support for social and economic development.