Against the background of the in-depth reform of vocational education, the construction of “dual-qualification and dual-competence” teaching teams has become increasingly prominent as a core link to promote the in-depth development of industry-education integration and improve the quality of technical and skilled talent training. Due to its strong practical characteristics, the nursing major has higher requirements for teachers’ “dual-qualification and dual-competence” (systematic theoretical teaching ability and clinical practical operation ability). Taking the Innovation Team for Education and Teaching of Nursing Professional Group of Guangzhou Huanan Business College as the research object, this paper systematically analyzes the practical paths constructed by the team in the construction of “dual-qualification and dual-competence”, such as the “internal training and external introduction” teacher optimization mechanism, the “school-hospital collaboration” practical training platform, and the “quantitative + qualitative” evaluation system. It also deeply discusses the structural contradictions faced in the construction process and the breakthrough strategies. The purpose is to provide a referenceable and promotable practical model for the construction of teaching staff in higher vocational nursing majors and contribute to the high-quality development of vocational education.