Objective: To explore the application effect of humanistic care in high-quality nursing services of the gastroenterology department. Methods: A total of 40 patients in the gastroenterology department admitted to our hospital from July 2024 to July 2025 were randomly divided into two groups. The experimental group (20 cases) received high-quality nursing services integrated with humanistic care, while the control group (20 cases) only received high-quality nursing services. Results: The scores of depression (SDS) and anxiety (SAS) in the control group were significantly higher than those in the experimental group; the understanding and mastery of dietary health knowledge in the control group were significantly lower than those in the experimental group; the satisfaction degree of the control group was significantly lower than that of the experimental group, and the differences between the groups were statistically significant (p < 0.05). Conclusion: The application of humanistic care in high-quality nursing services of the gastroenterology department can not only promote the rapid recovery of the body, but also improve patients’ compliance, effectively alleviate their negative emotions, guide patients to develop good dietary and living habits, and effectively improve their quality of life. Therefore, it has high clinical application value and is worthy of vigorous promotion and active reference.