Objective: To evaluate the effect of connotation construction control on antimicrobial use density (AUD) after the application of multiple strategic interventions. Methods: The experimental subjects were discharge data from Dehong Prefectural People’s Hospital, with the scope of this study spanning from January 2024 to December 2025. Comprehensive data from 2024 were included in the pre-intervention group, while comprehensive data from 2025 were included in the post-intervention group. A set of antimicrobial management systems was constructed, characterized by precision, standardization, and systematization, and multiple measures were implemented (including organizational system restructuring, information-based precise control, personalized indicator management, dynamic authorization and training of prescription rights, and multidisciplinary collaboration (MDT), etc.). The following indicators before and after the intervention were statistically analyzed and compared: AUD, antimicrobial use rate, etiological examination submission rate, and irrational prescription rate. Results: The post-intervention AUD was significantly lower than that before the intervention [(39.46 ± 2.08) DDDs/(100 person-days) vs (36.79 ± 0.22) DDDs/(100 person-days)], and the post-intervention antimicrobial use rate, proportion of special-grade antimicrobials used, and irrational prescription rate showed a lower trend compared to those before the intervention (p < 0.05); the etiological examination submission rate for therapeutic medications showed a higher trend compared to that before the intervention (p < 0.05). Conclusion: By focusing on connotation construction and implementing multiple strategic interventions, a shift from simple indicator control to precise diagnosis and treatment and rational medication use was achieved, significantly reducing the antimicrobial use density and improving the rationality of medication use.