Objective: To explore the application effect of the intelligent blood collection system in the outpatient blood collection of cancer specialized hospitals, and to provide a basis for improving the quality of outpatient blood collection nursing and the medical experience of patients. Methods: 136,274 blood specimens collected using the traditional manual blood collection mode from January to June 2020 were selected as the control group, and 176,989 blood specimens collected using the intelligent blood collection system from January to June 2021 were selected as the observation group. The blood collection process time, blood specimen quality, incidence of adverse events and patient satisfaction of the two groups were compared. Measurement data were expressed as ± s and analyzed by t-test. Counting data were expressed as cases (%), and the χ² test was used. A p value < 0.05 was considered statistically significant. Results: The registration and number collection time, specimen submission time and total process time of the observation group were significantly shorter than those of the control group (p < 0.001). The total error rate of blood samples decreased from 0.374% to 0.050%, and the error rate of blood collection tube usage decreased from 3.8% to 0.7%. The patient satisfaction rate increased from 88% to 96%, and the differences were statistically significant (p < 0.05). Conclusion: The intelligent blood collection system can optimize the outpatient blood collection process, shorten the service duration, reduce the error rate of specimens, improve the efficiency of nursing and patient satisfaction, and has good application value in the outpatient blood collection management of tumor specialized hospitals.