Objective: To construct a digital and intelligent nursing simulation teaching case library based on INACSL best practice standards, and to evaluate the effect of clinical teaching for nursing interns under this model. Method: The case library was established based on the best practice standards of INACSL. Sixty nursing interns were selected and divided into a control group and an experimental group. The control group received traditional teaching, while the experimental group received digital and intelligent case simulation teaching based on traditional teaching. The theoretical and operational assessment results, clinical practice ability, critical thinking ability, and teaching satisfaction of the two groups were compared. Results: The theoretical scores (86.52 ± 4.38) and operational scores (88.15 ± 3.96) of the experimental group were higher than those of the control group (79.63 ± 5.12, 80.24 ± 4.85) (p < 0.05). The total scores of clinical practice ability (178.65 ± 12.34) and critical thinking ability (298.42 ± 18.56) in the experimental group were higher than those in the control group (152.37 ± 15.68, 265.19 ± 20.43) (p < 0.05). The teaching satisfaction rate of the experimental group (96.7%) was higher than that of the control group (80.0%) (p < 0.05). Conclusion: The digital and intelligent nursing simulation teaching case library created based on INACSL best practice standards can improve the clinical practice ability, critical thinking ability and teaching satisfaction of nursing interns.