In recent years, with the deepening research on the pathogenesis of myocardial ischemia-reperfusion injury (MIRI) and the continuous development of acupuncture-moxibustion medicine, acupuncture, as a safe and effective non-pharmacological therapy, has shown unique advantages and promising prospects in the prevention and treatment of MIRI. MIRI refers to the pathophysiological process in which myocardial cell structural damage and dysfunction are aggravated when blood perfusion is restored after a period of myocardial ischemia, and it is one of the key factors affecting the clinical prognosis of acute myocardial infarction and other diseases. This paper first expounds the methodological innovation and standardization of acupuncture for MIRI, and then proposes the optimization of clinical application of acupuncture for MIRI, aiming to provide new ideas and practical evidence for improving the clinical prevention and treatment effect of MIRI, and promote the standardized application and popularization of acupuncture therapy in the field of cardiovascular disease rehabilitation.