Objective: To strengthen personnel management for a clean operating room and ensure an automatic, intelligent, and scientific workflow. Methods: The medical behavior management system has been implemented to monitor and manage medical personnel entering and exiting the operating room, so as to meet the standard requirements of the operating room. Results: The flow of personnel has been controlled effectively, the flow in and out of the operating room has been optimized, the management level of the operating room has improved, and the cost has been cut down. Conclusion: With the advent of the information age and the continuous improvement of the management system, the management of operating rooms has become more reasonable and humanized; the management mode, working environment, and the overall quality of nursing work in operating rooms have improved.