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28 July 2023
Exploring the Mechanism of Circ-vgll3 in Osteogenically Differentiated Human Bone Marrow Mesenchymal Stem Cells
Yajie Huo ,  Yu Mao ,  Fang Luo ,  Fengjiao Zhang ,  Lifang Xie ,  Xiaoke Zhang ,  Kai Liu ,  Ling Sun ,  Hongmei Liu ,  Lige Song ,  Huanhuan Wang ,  Zhiqiang Kang
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1 Department of Endocrinology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007, Henan Province, China,
2 Department of Endocrinology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007, Henan Province, China,
3 Department of Endocrinology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007, Henan Province, China,
4 Department of Endocrinology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007, Henan Province, China,
5 Department of Endocrinology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007, Henan Province, China,
6 Department of Endocrinology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007, Henan Province, China,
7 Department of Endocrinology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007, Henan Province, China,
8 Department of Endocrinology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007, Henan Province, China,
9 Department of Endocrinology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007, Henan Province, China,
10 Department of Endocrinology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007, Henan Province, China,
11 Department of Endocrinology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007, Henan Province, China,
12 Department of Endocrinology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007, Henan Province, China,
JCNR 2023 , 7(4), 151–158; https://doi.org/10.26689/jcnr.v7i4.5139
© 2023 by the Authors. Licensee Whioce Publishing, Singapore. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
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Abstract

Objective: To explore the mechanism of circRNA-vgll3 in osteogenic differentiation of human bone marrow mesenchymal stem cells. Methods: BMSCs cells were transfected with circRNA-vgll3, and divided into circRNA-vgll3 high-level group, circRNA-vgll3 low-level group, and negative control group (circRNA-vgll3 not transfected) according to the amount of transfection. The proliferation and apoptosis of BMSCs osteoblasts in each group were analyzed, and the alkaline phosphatase (ALP) activity, type I collagen gray value, bone morphogenetic protein 2 (BMP-2), Runx2 protein, and mRNA expression levels were detected. Results: The circRNA-vgll3 low-level group had a significant inhibitory effect on the proliferation of BMSCs osteoblasts, and the apoptosis rate of the circRNA-vgll3 low-level group was significantly higher than that of the circRNA-vgll3 high-level group ( P < 0.05 ); ALP activity, type I collagen gray value, BMP-2, Runx2 protein, and mRNA expression levels in the high-level circRNA-vgll3 group were significantly higher than those in the low-level circRNA-vgll3 group, and the difference was statistically significant (P < 0.05). Conclusion: Overexpression of circRNA-vgll3 can promote the osteogenic differentiation ability of BMSCs, while low expression of circRNA-vgll3 can inhibit the osteogenic differentiation ability of BMSCs. The main mechanism of action is that circRNA-vgll3 can affect osteogenic differentiation by regulating the Runx2 protein.

References
Zou J, Yang W, Cui W, et al, 2023, Therapeutic Potential and Mechanisms of Mesenchymal Stem Cell-Derived Exosomes as Bioactive Materials in Tendon-Bone Healing. J Nanobiotechnology, 21(1): 14. https://doi.org/10.1186/s12951-023-01778-6
Iaquinta MR, Lanzillotti C, Mazziotta C, et al, 2021, The Role of MicroRNAs in the Osteogenic and Chondrogenic Differentiation of Mesenchymal Stem Cells and Bone Pathologies. Theranostics, 11(13): 6573–6591. https://doi.org/10.7150/thno.55664
Song W, Bo X, Ma X, et al, 2022, Craniomaxillofacial Derived Bone Marrow Mesenchymal Stem/Stromal Cells (BMSCs) for Craniomaxillofacial Bone Tissue Engineering: A Literature Review. J Stomatol Oral Maxillofac Surg, 123(6): e650–e659. https://doi.org/10.1016/j.jormas.2022.06.002
Vulf M, Khlusov I, Yurova K, et al, 2022, MicroRNA Regulation of Bone Marrow Mesenchymal Stem Cells in the Development of Osteoporosis in Obesity. Front Biosci (Schol Ed), 14(3): 17. https://doi.org/10.31083/j.fbs1403017
Lin Z, Tang X, Wan J, et al, 2021, Functions and Mechanisms of Circular RNAs in Regulating Stem Cell Differentiation. RNA Biol, 18(12): 2136–2149. https://doi.org/10.1080/15476286.2021.1913551
Zhao Q, Liu X, Yu C, et al, 2022, Macrophages and Bone Marrow-Derived Mesenchymal Stem Cells Work in Concert to Promote Fracture Healing: A Brief Review. DNA Cell Biol, 41(3): 276–284. https://doi.org/10.1089/dna.2021.0869
Chen X, Xie W, Zhang M, et al, 2022, The Emerging Role of Non-Coding RNAs in Osteogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells. Front Cell Dev Biol, 10: 903278. https://doi.org/10.3389/fcell.2022.903278
Chu DT, Phuong TNT, Tien NLB, et al, 2020, An Update on the Progress of Isolation, Culture, Storage, and Clinical Application of Human Bone Marrow Mesenchymal Stem/Stromal Cells. Int J Mol Sci, 21(3): 708. https://doi.org/10.3390/ijms21030708
Wu S, Ohba S, Matsushita Y, 2023, Single-Cell RNA-Sequencing Reveals the Skeletal Cellular Dynamics in Bone Repair and Osteoporosis. Int J Mol Sci, 24(12): 9814. https://doi.org/10.3390/ijms24129814
Xiong Z, Yi P, Lin J, et al, 2021, Evaluation of the Efficacy of Stem Cell Therapy in Ovariectomized Osteoporotic Rats Based on Micro-CT and Dual-Energy X-Ray Absorptiometry: A Systematic Review and Meta-Analysis. Stem Cells Int, 2021: 1439563. https://doi.org/10.1155/2021/1439563
Arshad M, Jalil F, Jaleel H, et al, 2023, Bone Marrow Derived Mesenchymal Stem Cells Therapy for Rheumatoid Arthritis - a Concise Review of Past Ten Years. Mol Biol Rep, 50(5): 4619–4629. https://doi.org/10.1007/s11033-023-08277-9
Liu C, Xiao K, Xie L, 2022, Advances in the Regulation of Macrophage Polarization by Mesenchymal Stem Cells and Implications for ALI/ARDS Treatment. Front Immunol, 13: 928134. https://doi.org/10.3389/fimmu.2022.928134
Daniel M, Bedoui Y, Vagner D, et al, 2022, Pathophysiology of Sepsis and Genesis of Septic Shock: The Critical Role of Mesenchymal Stem Cells (MSCs). Int J Mol Sci, 23(16): 9274. https://doi.org/10.3390/ijms23169274
Miari KE, Williams MTS, 2023, Stromal Bone Marrow Fibroblasts and Mesenchymal Stem Cells Support Acute Myeloid Leukaemia Cells and Promote Therapy Resistance. Br J Pharmacol, online ahead of print. https://doi.org/10.1111/bph.16028
García-Sánchez D, González-González A, Alfonso-Fernández A, et al, 2023, Communication Between Bone Marrow Mesenchymal Stem Cells and Multiple Myeloma Cells: Impact on Disease Progression. World J Stem Cells, 15(5): 421–437. https://doi.org/10.4252/wjsc.v15.i5.421
Zong Q, Bundkirchen K, Neunaber C, et al, 2023, Are the Properties of Bone Marrow-Derived Mesenchymal Stem Cells Influenced by Overweight and Obesity? Int J Mol Sci, 24(5): 4831. https://doi.org/10.3390/ijms24054831
Liang C, Liu X, Yan Y, et al, 2022, Effectiveness and Mechanisms of Low-Intensity Pulsed Ultrasound on Osseointegration of Dental Implants and Biological Functions of Bone Marrow Mesenchymal Stem Cells. Stem Cells Int, 2022: 7397335. https://doi.org/10.1155/2022/7397335
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