Objective: To study the effect of the direct anti-human globulin test on the clinical efficacy of blood transfusion patients. Methods: 52 transfused patients were selected for this study, of which 26 cases with positive direct anti-human globulin tests were included in the positive group, and another 26 cases with negative direct anti-human globulin tests were included in the negative group. The apparent efficacy of the patients in the two groups after blood transfusion was compared. Results: After blood transfusion, the apparent efficacy of the negative group was significantly higher, P < 0.05; in the positive group, the proportion of the predominantly multi-antibody group was the highest; after blood transfusion, the post-transfusion apparent efficacy of the simple IgG group was higher than that of the multi-antibody group, P < 0.05; comparing the intensity of the different antibodies resulted in the 1+ group, and the 3+ to 4+ groups were significantly lower after blood transfusion, P < 0.05. Conclusion: The use of the direct antiglobulin test in transfused patients showed that patients with positive results would have better clinical efficacy. Direct anti-human globulin tests will have an impact on the clinical efficacy of blood transfusion in patients with positive results, so it is very important to carry out a direct anti-human globulin test on blood transfusion patients.