Isolation and Experimental Study on Bacteriostasis of Bacillus haynesii

² Foshan City Nanhai Eastern Along Pharmaceutical Co., Ltd, Foshan 528234, Guangdong Province, China; Department of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, Guangdong Province, China,

³ Foshan City Nanhai Eastern Along Pharmaceutical Co., Ltd, Foshan 528234, Guangdong Province, China,

⁴ College of Animal Science and Technology, Foshan University, Foshan 528225, Guangdong Province, China; Foshan City Nanhai Eastern Along Pharmaceutical Co., Ltd, Foshan 528234, Guangdong Province, China,

⁵ Department of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, Guangdong Province, China,

JCNR 2024 , 8(7), 219–227; https://doi.org/10.26689/jcnr.v8i7.7874

© 2024 by the Authors. Licensee Whioce Publishing, Singapore. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )

Abstract

This experiment aims to isolate and inhibit three bacteria strains to provide candidate strains for the development and application of probiotics. Using bacterial morphological identification, 16S rDNA sequence alignment, and genetic evolution analysis, three strains were identified as Bacillus haynesii, named HP01, HD02, and HK03. Through biosurfactant activity tests, C-TAB tests, hemolysis tests, and antibacterial activity analyses, the results showed that all three strains of B. haynesii exhibited significant biosurfactant activity. Additionally, the solutions of the three strains demonstrated a pronounced antibacterial effect on Staphylococcus aureus. The resistance and safety of commonly used drugs were evaluated using the tablet diffusion method and a mouse feeding test. The results indicated that the three strains were not resistant to commonly used antibacterial drugs, and the oral bacterial solution was not pathogenic and had high safety in mice. The study concluded that all three B. haynesii strains met the basic conditions for use, with B. haynesii HP01 being the most promising candidate.

References

Dunlap CA, Schisler DA, Perry EB, et al., 2017, Bacillus swezeyi sp. nov. and Bacillus haynesii sp. nov., Isolated from Desert Soil. Int J Syst Evol Microbiol, 67(8): 2720–2725. https://doi.org/10.1099/ijsem.0.002007

Rajitha K, Nancharaiah YV, Venugopalan VP, 2020, Acid Soluble Extracellular Matrix Confers Structural Stability to Marine Bacillus haynesii Pellicle Biofilms. Colloids and Surfaces B: Biointerfaces, 194: 111160. https://doi.org/10.1016/j.colsurfb.2020.111160

Maurya A, Kumar R, Yadav P, et al., 2022, Biofilm Formation and Extracellular Polymeric Substance (EPS) Production by Bacillus haynesii and Influence of Hexavalent Chromium. Bioresour Technol, 352: 127109. https://doi.org/10.1016/j.biortech.2022.127109

Abdelgalil SA, Kaddah MMY, Duab MEA, et al., 2022, A Sustainable and Effective Bioprocessing Approach for Improvement of Acid Phosphatase Production and Rock Phosphate Solubilization by Bacillus haynesii Strain ACP1. Sci Rep, 12(1): 8926. https://doi.org/10.1038/s41598-022-11448-6

Marin-Sanhueza C, Echeverria-Vega A, Gomez A, et al., 2022, Stress Dependent Biofilm Formation and Bioactive Melanin Pigment Production by a Thermophilic Bacillus Species from Chilean Hot Spring. Polymers (Basel), 14(4): 680. https://doi.org/10.3390/polym14040680

Wang W, Zhu L, Lv P, et al., 2018, Novel Candy-like Cu4O3 Microstructure: Facile Wet Chemical Synthesis, Formation Mechanism, and Good Long-Term Antibacterial Activities. ACS Appl Mater Interfaces, 10(43): 37287–37297. https://doi.org/10.1021/acsami.8b14470

Zhou S, Gu Z, Zhang R, et al., 2023, Identification and Antimicrobial Test of Bacillus velezensis. Guangdong Journal of Animal and Veterinary Science, 48(4): 30–34 + 41.

Palit K, Das S, 2024, Cellulolytic Potential of Mangrove Bacteria Bacillus haynesii DS7010 and the Effect of Anthropogenic and Environmental Stressors on Bacterial Survivability and Cellulose Metabolism. Environ Res, 252(Pt 1): 118774. https://doi.org/10.1016/j.envres.2024.118774

Kosarsoy Agceli G, 2023, Similarities and Differences of Nano-Sized Levan Synthesized by Bacillus haynesii at Low and High Temperatures: Characterization and Bioactivity. Int J Biol Macromol, 253(Pt 3): 126804. https://doi.org/10.1016/j.ijbiomac.2023.126804

Rahman MM, Paul SI, Rahman A, et al., 2022, Suppression of Streptococcosis and Modulation of the Gut Bacteriome in Nile Tilapia (Oreochromis niloticus) by the Marine Sediment Bacteria Bacillus haynesii and Advenella mimigardefordensis. Microbiol Spectr, 10(6): e0254222. https://doi.org/10.1128/spectrum.02542-22

Previous article in this issue

Next article in this issue