Objective: To analyze the distribution and blood type characteristics of unpaid blood donors in Linxia area from 2012 to 2021, provide the basis for the strategy of recruiting safe blood sources in the future, ensure sufficient and safe blood supply, and promote the sustainable development of unpaid blood donation in this area. Methods: Collect the data of unpaid blood donors in Linxia area, and make statistics, comparison and analysis based on the query information of the fourth generation safe blood transfusion standardization system and the free blood donation registration form. Results: The total number of blood donors was 151131 and the ratio of male to female blood donors was 2.61∶1. People aged 18–45 were the main blood donors, accounting for 80.78% of all unpaid blood donors. 37.19% of people with junior high school education or below; Farmers, other professionals and civil servants were the main subjects of blood donation, accounting for 15.74%, 14.98% and 12.12%, respectively. The blood group distribution was O > B > A > AB, and the negative rate was 8.2%. Blood type O was the most common in Hezheng County (34.08%). The distribution of blood type B in Kangle County (31.99%), blood type A in Yongjing County (29.58%), blood type AB in Dongxiang County (10.18%). Conclusion: The unpaid blood donors in Linxia area have significant characteristics in terms of gender, age, occupation, education level, etc. to master the distribution characteristics of the unpaid blood donors and blood types in Linxia area is helpful to carry out targeted publicity and recruitment of unpaid blood donation and blood collection, and is of great significance to continuously strengthen the contingent of unpaid blood donors and fully guarantee the clinical blood demand under the new situation.