Objective: To design a preoperative checklist for coronary interventional surgery based on multidisciplinary collaboration, and verify its effect on improving the completeness rate of preoperative preparation, surgical punctuality, and medical staff satisfaction. Methods: A multidisciplinary team was established involving the Department of Cardiology, Anesthesiology, Clinical Laboratory, and Interventional Surgery. Referring to the latest clinical guidelines and nursing standards, a preoperative checklist covering 8 core modules and 61 sub-items was formulated, including “1 day before surgery + day of surgery” and “doctor + nurse + patient + family member”. A total of 213 patients undergoing coronary interventional surgery in our hospital from August 2023 to February 2024 (observation group) were selected to apply the checklist. The completeness rate of preoperative preparation and the on-time operation start rate were compared with 197 patients (control group) before the checklist application. The satisfaction of 34 medical staff was evaluated using a Likert 5-point scale. Results: The overall completeness rate of preoperative preparation in the observation group reached 95.72% (78.17% in the control group), among which the completeness rates of 6 sub-items such as medication, health education, and laboratory examinations reached 100%, and the completeness rate of consumables and equipment preparation was 97.1%; the on-time operation start rate was 97.3%; the satisfaction of the medical team with the checklist was 94.12%, which was significantly higher than that of the control group (82.61%), with statistically significant differences (p < 0.05). Conclusion: The preoperative checklist for coronary interventional surgery designed through multidisciplinary collaboration can significantly improve the quality of preoperative preparation and surgical efficiency, and is highly recognized by the medical team, providing practical reference for perioperative safety management of interventional diagnosis and treatment.