Objective: To study the application effect of bundle nursing strategy in the maintenance of difficult blood vessels for long-term hemodialysis patients. Methods: A prospective study was conducted on 40 long-term hemodialysis patients admitted from January 2024 to January 2025. The patients were randomly divided into a control group (20 cases) and an observation group (20 cases) using computer random sampling. The control group received routine nursing for difficult blood vessels, while the observation group received a bundle nursing strategy for difficult blood vessels. The self-care ability, complication rate, and nursing satisfaction were compared between the two groups. Results: After nursing, the self-care ability score of the observation group was higher than that of the control group (P < 0.05). The complication rate in the observation group was lower than that in the control group (P < 0.05). The nursing satisfaction in the observation group was higher than that in the control group (P < 0.05). Conclusion: The application of the bundle nursing strategy in the maintenance of difficult blood vessels for long-term hemodialysis patients is beneficial for improving self-care ability, reducing complications, and increasing patient satisfaction with nursing services.