With the in-depth development of emerging engineering education, higher education has put forward higher requirements for the cultivation of engineering professionals. Professional practical courses are an important part of undergraduate teaching plans, aiming to improve students’ practical abilities and their ability to comprehensively apply professional knowledge. To further explore teaching reform and improve the diversified curriculum assessment and evaluation system, this paper proposes a series of teaching reform methods for professional practical courses. It reforms the content and teaching methods of professional practical courses. On the basis of introducing practical application scenarios and cutting-edge technologies, it adds interdisciplinary modules, makes full use of internal and external school resources to break traditional disciplinary boundaries, and integrates interdisciplinary content from cutting-edge fields such as artificial intelligence, big data, and biotechnology. This enhances students’ understanding of industry needs, practical operation capabilities, and awareness of multi-disciplinary integration. Secondly, it promotes a student-centered teaching model, increases students’ opportunities for independent participation, and cultivates their independent thinking and innovation abilities. At the same time, it reforms the evaluation system: by comprehensively considering students’ performance and achievements in the process of professional practical courses, it measures students’ abilities more comprehensively through a multi-dimensional evaluation system. This study provides new ideas for the teaching of professional practical courses.