Objective: To systematically retrieve, evaluate, and summarize the evidence regarding tumor-free techniques, thereby providing a foundation for standardizing nursing practices in malignant tumor surgery. Methods: According to the evidence-based “6S” model, a comprehensive search of literature pertaining to tumor-free technology was conducted using both Chinese and English databases. The time frame for this search extended from the inception of the database up until December 2025. Results: A total of 21 articles were included in this review. Among these, there was 1 clinical decision, 7 guidelines, 3 expert consensus documents, 6 systematic reviews, 1 randomized controlled trial, and 3 quasi-experiments. The topics covered encompassed preoperative personnel preparation, establishment of isolation areas, incision protection, body cavity exploration, management of carbon dioxide pneumoperitoneum, puncture needle management, tumor resection techniques, irrigation fluid management practices, surgical instrument handling protocols, and tumor specimen management. In total, there were 10 distinct topics with an aggregate of 27 items addressed. Conclusion: The evidence presented in this study serves as a valuable reference for the standardization of tumor-free techniques. It aims to enhance operating room nurses’ awareness of these techniques and mitigate the risk of iatrogenic tumor implantation and metastasis during surgical procedures.