The Spatial Location of Chromosomes in Dividing Cells and the Relative Stability of Chromosome Spatial Structure

¹ Jiangsu Institute of Hematology, National Clinical Research Center for Hematologic Diseases, NHC Key Laboratory of Thrombosis and Hemostasis, The First Affiliated Hospital of Soochow University, Suzhou 215008, Jiangsu Province, China,

² The Second Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210000, Jiangsu Province, China,

³ Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210000, Jiangsu Province, China,

⁴ The Second Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Second Chinese Medicine Hospital, Nanjing 210000, Jiangsu Province, China,

⁵ Department of Epidemiology and Health Statistics, School of Public Health, Southeast University, Nanjing 210000, Jiangsu Province, China,

⁶ Experiment Center for Science and Technology, Nanjing University of Chinese Medicine, Nanjing 210000, Jiangsu Province, China,

PAR 2025 , 9(2), 83–91; https://doi.org/10.26689/par.v9i2.9965

© 2025 by the Authors. Licensee Whioce Publishing, Singapore. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )

Abstract

The stability and evolution of human genetics depend on chromosomes and chromosome-chromosome interactions. We wish to understand the spatial location of chromosomes in dividing cells in order to understand the relationship between chromosome-chromosome interactions and to further investigate the role of chromosomes and their impact on cell biological behavior. In this study, we explored the relative spatial positional relationships of chromosomes [t(9;22) and t(15;17)] in B-ALL cells by using the three-dimensional DNA fluorescent in situ hybridization (3D-FISH) method. The results showed that chromosomes [t(9;22) and t(15;17)] showed relatively stable spatial relationships. The relative stability of the spatial location of chromosomes in dividing cells may be relevant to disease.

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