Study on the Relationship between Secondary Metabolites and Resistance to Anthracnose in Stylosanthes

¹ Analysis and Testing Center, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Quality and Safety for Tropical Fruit and Vegetable Products, Haikou 571101, Hainan Province, China,

² Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 570100, Hainan Province, China; Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan Province, China,

³ Hainan Food Inspection and Testing Center, Haikou 570100, Hainan Province, China,

⁴ Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 570100, Hainan Province, China,

ssr 2024 , 6(11), 88–105; https://doi.org/10.26689/ssr.v6i11.8779

Abstract

Stylosanthes is an excellent tropical leguminous forage. Anthracnose caused by Colletotrichum gloeosporioides is a major limiting factor in Stylosanthes production, which can lead to reduced forage and seed production and decreased quality. How to quickly screen resistant parents and varieties of Stylosanthes that are resistant to anthracnose has become an urgent problem to be solved in production. In this study, the high-performance liquid chromatography detection technology was established for simultaneous analysis of the content of salicylic acid, triethyl orthobenzoate, indazole, and soyasapogenol B in Stylosanthes leaves. The technology was further applied in the analysis of the relationship between secondary metabolites and resistance to anthracnose in 105 accessions of Stylosanthes. The result shows that salicylic acid is the most important factor for resistance to anthracnose. By detecting the content of salicylic acid in Stylosanthes leaves, a rapid screening technology for anthracnose-resistant parents and varieties of Stylosanthes was established, and 5 accessions of Stylosanthes with high resistance to anthracnose were screened. This study is of great significance to the Stylosanthes germplasm preservation and identification and its breeding.

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