This study surveyed 324 female college students and tested them using the perceived stress scale (PSS), the college student network moral misconduct scale (NABS), and the interaction anxiety scale (IAS). The research results indicate that: First, there is a significant positive correlation between perceived stress, social anxiety, and female college students’ online moral misconduct behavior; Second, the direct effect of perceived stress on female college students’ online moral misconduct is significant (B = 0.987, P < 0.01); Third, the mediating effect of perceived stress on female college students’ online moral misconduct through social anxiety is significant (B = 0.693, P < 0.01). The research conclusion is that social anxiety plays a partial mediating role in the impact of perceived stress on female college students’ online moral misconduct.