The cultural construction of border ports bears a close bearing on national image, frontier stability, and cultural security. Against the backdrop of in-depth implementation of the governance philosophy that governance of the country hinges on effective border administration, researching the cultural development of land border ports carries profound practical implications. As China’s pivotal opening gateway toward South and Southeast Asia, Yunnan’s border port cultural construction exerts far-reaching significance for boosting cross-border people-to-people exchanges and facilitating the Belt and Road Initiative. Within the nationwide systematic project of constructing a ten-thousand-li cultural corridor along national frontiers, Yunnan takes the lead in putting forward the development conception of national gate culture and has carried out bold practical explorations and attained tangible achievements in port cultural development. Relevant investigations reveal that the infrastructure supporting border port culture has been remarkably upgraded, and cross-border ethnic cultural activities have grown increasingly affluent in recent years. Nevertheless, prominent bottlenecks still linger, including insufficient integration of scattered cultural resources, imperfect cross-border cultural governance frameworks, latent cultural security hazards, and underdeveloped brand cultivation and digital communication mechanisms. Targeted at such existing drawbacks, this paper puts forward a five-project practical framework to advance the high-quality cultural progression of land border ports.