Objective: To analyze the application value of the comprehensive guided labor nursing model in improving maternal negative emotions. Methods: Eighty cases of mothers admitted to the hospital from June 2022 to June 2023 were sampled and randomly grouped, with comprehensive guided labor nursing intervention included in Group A and conventional care included in Group B. Emotion scores, labor and delivery, pregnancy outcomes, and nursing satisfaction were compared. Results: Maternal self-rating anxiety (SAS) and depression (SDS) scale scores were lower in Group A than in Group B (P < 0.05). Maternal visual analog scores (VAS) and postpartum hemorrhage were lower in Group A than in Group B (P < 0.05). Additionally, the duration of each stage of labor was shorter than in Group B (P < 0.05). The incidence of adverse pregnancy outcomes was lower in Group A than in Group B, and the rate of spontaneous deliveries was higher in Group A compared to Group B (P < 0.05). Furthermore, nursing care satisfaction was higher in Group A than in Group B (P < 0.05). Overall, Group A had a higher rate of maternal nursing satisfaction than Group B (P < 0.05). Conclusion: Comprehensive guided labor nursing can shorten the duration of labor, reduce postpartum hemorrhage, and decrease the rate of adverse pregnancy outcomes, thereby contributing to the improvement of maternal mood. This approach proves to be efficient and feasible in clinical practice.