This case report presents a 23-year-old female patient who developed eyebrow and follicular damage following the use of cosmetic products. The patient experienced gradual eyebrow thinning and hair loss after applying eyebrow powder. Physical examination was unremarkable, while dermoscopy revealed hair breakage, follicular damage, and the presence of the black dot sign. Reflectance confocal microscopy showed infiltration of inflammatory cells. Patch testing was strongly positive. After excluding other systemic conditions, a diagnosis of cosmetic-induced follicular damage was established based on the clinical findings and test results. Discontinuation of the cosmetic product led to eyebrow regrowth. This case underscores the potential risks of cosmetic ingredients to hair health and highlights the utility of non-invasive diagnostic techniques for clinical evaluation.