Objective: To explore the application and effectiveness of PDCA cycle management in outpatient blood collection room management. Methods: A total of 80 patients in the outpatient blood collection room of the hospital from June 2023 to June 2024 were selected as the study subjects and randomly divided into a control group and an observation group, with 40 patients in each group. The control group implemented conventional nursing management, while the observation group implemented PDCA cycle management on top of routine care. Blood collection outcomes, the incidence of disputes, adverse reactions, blood collection waiting times, nursing quality, and patient satisfaction were compared between the two groups. Results: The observation group showed significantly better blood collection outcomes compared to the control group, with a higher success rate of venipuncture (P < 0.05). The total incidence of adverse reactions in the observation group was notably lower than in the control group (P < 0.05). Additionally, the observation group exhibited shorter blood collection waiting times, higher nursing quality scores, and greater patient satisfaction, with all differences showing high statistical significance (P < 0.001). Conclusion: The application of PDCA cycle management in the outpatient blood collection room demonstrated remarkable effectiveness. By leveraging the cycle of planning, execution, checking, and action, it optimized blood collection processes, improved efficiency and service quality, and enhanced patient satisfaction, making it highly worthy of clinical promotion and application.