Objective: To evaluate the therapeutic efficacy of alanyl-glutamine (Ala-Gln) in the nutritional support of sepsis. Methods: 120 cases of sepsis patients admitted to the hospital in the past three years were selected and grouped by randomization method, Group A was treated with Ala-Gln, and Group B was treated with conventional nutritional support therapy, and the therapeutic effects were compared. Results: Before treatment, there was no difference in nutritional indexes, immune function indexes, and inflammatory factors between the two groups (P > 0.05). After treatment, the nutritional indexes of Group A were higher than those of Group B, the immune function indexes were higher than those of Group B, and the inflammatory factors were lower than those of Group B (P < 0.05). Except for mortality, the regression of group A was better than that of group B (P < 0.05). Conclusion: Ala-Gln can improve the nutritional indexes of septicemia patients during the treatment period, enhance their immune function, reduce the inflammatory response of the body, and promote the regression of the disease.