University education is an important cornerstone of national development and social progress. Our university, a national key university with strengths in forestry disciplines, has been approved by the State Council as a “Double First-Class” construction university. To cultivate top-notch innovative talents in biology, high-quality teaching reform is urgently needed. Developmental Biology is a core course for the biological science major at our university. However, due to its high difficulty, strong interdisciplinary nature, and short establishment time, there are many teaching pain points, especially in the experimental course, such as outdated knowledge systems and unreasonable teaching design. These issues significantly hinder the achievement of the university’s talent cultivation goals. Therefore, it is imperative to adapt to the new talent cultivation model, reform the curriculum system, teaching content, and teaching methods, and put these reforms into practice. Through such course reforms, we aim to improve teaching effectiveness and talent cultivation quality to meet the requirements of “Double First-Class” university construction.