This paper discusses the teaching objectives and contents, the implementation of teaching mode, methods, and the results of course construction of the course “Special Topic on Application of Artificial Intelligence,” which is set up for students majoring in artificial intelligence in Northwest A&F University. The course aims to comprehensively improve students’ vision, thinking and ability to solve professional complex problems by using artificial intelligence technology. The course covers the application of artificial intelligence technology, such as generative artificial intelligence, multi-objective reinforcement learning, and multi-modal machine learning in marketing, power grid, medical, and agricultural industries. The teaching of the course mainly adopts the mode of online and offline mixing, the integration of science and practice, and the cooperation of school and enterprise, and combines the methods of special report, exchange and discussion, and project report. The teaching feedback shows that students generally hold a positive attitude towards the course content, teaching mode and method, which provides useful reference for the construction and practice of the course in other universities and majors.