As a core cutting-edge technology in the field of life sciences, genetic engineering is a key course for the biotechnology major that connects basic theories with industrial applications, and its teaching quality is directly related to the cultivation of high-quality professional talents. Under the dual background of the construction of “New Engineering” and the certification of normal majors, the rapid development of the biotechnology industry puts forward higher requirements for students’ practical and innovative abilities, and the reform of basic education also poses new challenges to the disciplinary teaching transformation ability of normal biology teachers. However, the traditional teaching of the genetic engineering course has prominent problems, such as lagging knowledge and a disconnection between theory and practice, which makes it difficult to adapt to the talent training needs of the new era. Therefore, promoting the innovation of the course teaching mode has become an urgent task for professional education reform. Based on the talent training objectives of the biotechnology major (including normal direction), this paper integrates four core dimensions: AI empowerment, integration of theory and practice, industry-education collaboration, and literacy-oriented education, and constructs a systematic teaching reform plan, aiming to solve the current teaching problems, improve the course education quality, and provide practical reference for the teaching reform of similar courses.